A total of 290 climate change activists have been arrested for blocking roads in central London, amid protests aimed at shutting down the capital.
A second day of disruption is under way after Extinction Rebellion campaigners camped overnight at Waterloo Bridge, Parliament Square and Oxford Circus.
Police said 500,000 people had been affected by the diversion of 55 bus routes in London.
The Met said 290 people had been arrested by 21:30 BST on Tuesday.
Three men and two women, in their 40s and 50s, arrested on suspicion of criminal damage at Shell's headquarters on Monday, have since been released under investigation.
The majority of the other protesters detained have been held on suspicion of public order offences.
Campaigners have been ordered to restrict their protests to Marble Arch after they caused . That order will continue until 21:10 on Friday.
Ch Supt Colin Wingrove said: "Ongoing demonstrations are causing serious disruption to public transport, local businesses and Londoners who wish to go about their daily business."
Motorists face gridlocked traffic on a number of alternative routes, such as Westminster Bridge and Blackfriars Bridge.
Transport for London warned bus users that routes would remain on diversion or terminate early.
Mayor of London Sadiq Khan said although he "shared the passion" of the activists, he was "extremely concerned" about plans some protesters had to disrupt the London Underground on Wednesday.
He said it was "absolutely crucial" to get more people to use public transport to tackle climate change.
"Targeting public transport in this way would only damage the cause of all of us who want to tackle climate change, as well as risking Londoners' safety, and I'd implore anyone considering doing so to think again," he said.
Extinction Rebellion said it wanted to "shut down London" until 29 April in a series of protests.
It called for "reinforcements" to help maintain the roadblock at Waterloo Bridge.
Organisers said protests had been held in more than 80 cities across 33 countries, including in Scotland where 150 protesters have .Skip Twitter post by @BBCTomEdwards Report End of Twitter post by @BBCTomEdwards
Hundreds of protesters tried to hinder the police effort to move them on, including four who glued and chained themselves under a lorry parked on the bridge.
Ben Moss, 42, from Islington, north London, said he had glued himself to the bars of the lorry as "personal action to the moral issue of the climate crisis and ecological collapse".
"I'm doing this because I want the government to do something. I've got a week off work - if more is necessary I can make my excuses," he added.
On Monday, a pink boat was parked in the centre of Oxford Circus where some activists locked their arms together with makeshift devices, while oil company Shell's headquarters on Belvedere Road were vandalised.
What is Extinction Rebellion?
Since its launch last year, members have shut bridges, poured buckets of fake blood outside Downing Street, blockaded the BBC and stripped semi-naked in Parliament.
: for the government to "tell the truth about climate change", reduce carbon emissions to zero by 2025, and create a citizens' assembly to oversee progress.
Controversially, the group is trying to get as many people arrested as possible.
One of the group's founders, Roger Hallam, believes that mass participation and civil disobedience maximise the chances of social change.
But critics say they cause unnecessary disruption and waste police time when forces are already overstretched.
The second day of action included speeches at Parliament Square about how to tackle climate change.Skip Twitter post by @ExtinctionR Report End of Twitter post by @ExtinctionR
Many people trying to travel across London criticised the disruption, while others said the vandalism was "disgusting".
Peter Newport said on Twitter : "I agree with freedom of speech but if I can't get to work it's costing me money."Skip Twitter post by @sonorabuck Report End of Twitter post by @sonorabuck Skip Twitter post by @cubanpete70 Report End of Twitter post by @cubanpete70 Skip Twitter post by @ DavidBroad Report End of Twitter post by @ DavidBroad
At the scene: Shamaan Freeman-Powell, BBC News
Oxford Circus is usually one of the busiest crossroads in London, but only scores of protesters and bemused onlookers can be found in the middle of the road today.
Food stalls offering free porridge, and clothing lines for dirty laundry have been erected.
Children as young as six are making use of the freshly-drawn hopscotch, running around the tents and flying colourful banners.
One campaigner, who attended the protest with her two children, says she was protesting for the people who are "the most vulnerable, and least responsible for climate change".
Her nine-year-old daughter says she wishes her school taught her more on the issue.
Most protesters say the police have been encouraging - despite the number of arrests - although taxi drivers and shoppers complained of the disruption.
The government said it shared "people's passion" to combat climate change and "protect our planet for future generations".
The Department for Business, Energy & Industrial Strategy said the UK had cut its emissions by 44% since 1990.
A spokesman said: "We've asked our independent climate experts for advice on a net zero emissions target and set out plans to transition to low emission vehicles and significantly reduce pollution through our Clean Air Strategy "
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